Clinical Correlation of a Clostridium difficile Testing Algorithm Reflexing PCR Positive Specimens to Toxin Enzyme Immunoassay (EIA)
نویسندگان
چکیده
1287. Differences in Clinical Characteristics and Outcomes of Patients with Community-Onset Clostridium difficile Infection who Tested Positive by EIA Compared with NAAT through a Two-Step Algorithm Erin C. Phipps, DVM, MPH; Nicole Kenslow, MPH; Ioan Belovarski, MD, MPH and Emily B. Hancock, MS; New Mexico Emerging Infections Program, University of New Mexico, Albuquerque, New Mexico, University of New Mexico School of Medicine, Albuquerque, New Mexico
منابع مشابه
Evaluation of a new enzyme immunoassay for Clostridium difficile toxin A.
AIM To evaluate a new enzyme immunoassay (EIA) method for detection of Clostridium difficile toxin by comparing it to cytotoxicity assay. To investigate the nature of false negative and false positive EIA results by evaluating clinical and therapeutic parameters. METHODS 737 consecutive diarrhoeal specimens collected from patients clinically suspected of having C difficile colitis were tested...
متن کاملComparison of analytical and clinical performance of three methods for detection of Clostridium difficile.
CONTEXT Diagnostic laboratory testing for Clostridium difficile infection has undergone considerable and rapid evolution during the last decade. The ideal detection method(s), which should exhibit high analytical and clinical sensitivity and specificity, remains undefined. OBJECTIVE We sought to evaluate the analytical and clinical performance characteristics of three methods for the laborato...
متن کاملClostridium difficile testing in the clinical laboratory by use of multiple testing algorithms.
The incidence of Clostridium difficile infection (CDI) has risen almost 3-fold in the United States over the past decade, emphasizing the need for rapid and accurate tests for CDI. The Cepheid Xpert C. difficile assay is an integrated, closed, nucleic acid amplification system that automates sample preparation and real-time PCR detection of the toxin B gene (tcdB). A total of 432 stool specimen...
متن کاملDiagnosis of Clostridium difficile infection.
TO THE EDITOR—Despite our long-standing interest in earlier tests for Clostridium difficile toxin [1], we believe that polymerase chain reaction (PCR) technology should simply replace other algorithms for the diagnosis of C. difficile infection, as recently outlined in Clinical Infectious Diseases by Kufelnicka and Kirn [2]. We studied 122 consecutive specimens submitted for C. difficile testin...
متن کاملCost-effectiveness of a modified two-step algorithm using a combined glutamate dehydrogenase/toxin enzyme immunoassay and real-time PCR for the diagnosis of Clostridium difficile infection.
The analytical performance and cost-effectiveness of the Wampole Toxin A/B EIA, the C. Diff. Quik Chek Complete (CdQCC) (a combined glutamate dehydrogenase antigen/toxin enzyme immunoassay), two RT-PCR assays (Progastro Cd and BD GeneOhm) and a modified two-step algorithm using the CdQCC reflexed to RT-PCR for indeterminate results were compared. The sensitivity of the Wampole Toxin A/B EIA, Cd...
متن کامل